CoolLED pE-800fura- Supercharged calcium imaging and beyond

Description

Building on the successful pE-800 Illumination System, the pE-800^fura offers the most comprehensive calcium imaging system available. It also enhances a range of applications from pH monitoring and optogenetics to everyday fluorescence microscopy, combining lightning-fast switching with eight individually controllable channels.

Eight powerful and efficient LEDs offer spectral coverage from 340-635 nm, tailored to all popular calcium indicators, pH indicators, opsins, as well as fluorophores for everyday fluorescence ranging from Fura-2 to Cy5.

Individually controlling all eight channels is achieved via USB, TTL or analogue for easily matching each LED to the selected calcium indicator or fluorophore set.

Unlike lasers, the broader spectral output of LEDs can be refined by the use of optical filters in order to excite a range of dyes, expanding the coverage of the pE-800^fura. The ability to customise the wavelength output with both wavelength switching and optical filters means experiments are no longer limited by the light source, even as requirements evolve or new dyes emerge in the future.

Offering high-speed wavelength selection across the spectrum, the pE-800^fura presents the first direct LED upgrade to traditional monochromator solutions with all the benefits of LED illumination.

Accelerating calcium analysis

Industry-leading <7 µs TTL triggering is a breakthrough for ratiometric fluorescence applications such as calcium measurements, maximising the accuracy of measuring ratio pairs. For live cell imaging, capturing dynamic events is possible with the highest temporal resolution and even without software, thanks to precise hardware synchronisation via TTL input for each channel and compatibility with the pE-6501-8-FR (USB controlled TTL trigger box).

The pE-800 range has two models-

Features-

• Broad spectrum from 340-635 nm tailored to popular calcium indicators and fluorophores
• 8 selectable channels offer versatility with any channels selectable simultaneously at 100%
• Industry-leading <7 µs TTL triggering for high-speed analysis
• Affordable automation with Sequence Runner and a single TTL-out
• Removable inline excitation filter holder: no moving parts for high-speed imaging
• Software (USB and RS-232), TTL, and analogue control options for optimised illumination
• Optimised irradiance control in 1% steps (2-100%) and fine control from 0-2%
• Light delivery via liquid Light Guide for maximum compatibility
• Stable illumination minimises noise for precise calcium analysis and reduced false positives
• High signal-to-noise for crisp images and quality data with less calcium indicator, reducing toxicity and cost
• UV optimised optics maximises irradiance for Fura-2 and similar indicators

Suitable for most Leica, Olympus, Nikon and Zeiss microscopes- please ask us about the adapters needed.