The CoolLED
pE-300ultra is a highly controllable fluorescence microscopy Illumination System which offers intense, broad-spectrum LED illumination for imaging most common fluorophores. Fast events can be captured with microsecond switching via multiple TTL inputs, and the ability to mount inline excitation filters allows fast, flexible filter configurations.
The
pE-300ultra is the most controllable member of the pE-300 Series for LED microscope lighting. In addition to the many features of the
CoolLED pE-300
lite and the pE-300
white, the system offers precise control over wavelength irradiance and shuttering. Until now these benefits have only been accessible to users of high end, highly flexible LED microscope illumination systems such as the CoolLED pE-4000.
It allows adjustment of output in 1% steps, giving precise control. The broad spectrum covers everyday fluorophores: DAPI, CFP, Aqua, FITC, TRITC, TxRed, Cy5 and many more.
Direct fit or liquid light guide
There are two pE-300
ultra configuration options: direct fit or liquid light guide. The direct fit variant delivers higher irradiance and reduces the cost to buy and run thanks to fewer consumables.
Capturing high-speed events
The
pE-300ultra offers triggering from multiple TTL inputs which, coupled with the ability to mount inline excitation filters, provides microsecond switching of pre-filtered excitation light. This, when paired with today’s high performance multi-band filter sets, facilitates imaging traditionally done using a white light source and a filter wheel, with all the benefits of LED, and most excitingly at speeds not previously so affordable.
Fast multi-channel imaging
Fluorophores can be viewed either individually or in combination, without filter cube changes. This makes the
pE-300ultra ideal for use with multi-band filter sets as the screening process can be simplified when fewer filter cubes are used. Independent control of the three LED channels means that the user can control the level of excitation of each fluorophore independently on a multi-stained sample, potentially removing the need for single-band filter sets altogether.
Sequence runner for multi-channel excitation
It is now possible to run through a sequence of excitation channels using a camera or other external hardware which has only a single TTL-out, thanks to the Sequence Runner mode. Complex illumination protocols can now be run at high speeds and without the need specialised and costly hardware. The order of each fluorophore to be captured is defined via the simple Control Pod, with the
pE-300ultra Light Source accepting a single TTL output from the experiment set-up’s camera to initiate the step-through of a sequence of excitation channels. This feature is independent of the individual channel TTL inputs on the Light Source.
Energy efficiency for cost-saving sustainability
When it comes to making labs safer and more sustainable, LED microscope lighting is an attractive option when compared to old toxic mercury or metal halide lamps. All three channels of the
pE-300ultra at full power use just 60 Watts, which is even lower than other LED technology which uses 120 to 350 Watts.
Features
- Clean bright illumination across the spectrum – excites common fluorescent stains
- Specify for existing single and multi-band filter sets – no need to buy new filters
- Instant on/off – No shutters required, no warm up or cool down
- Simple to fit, simple to use – no alignment, a once only adjustment
- Stable & repeatable – reliable and consistent results
- Wide range of microscope adaptors – fits most microscopes
- Precise irradiance control in 1% steps (0-100%) – no ND filters required
- Excellent uniformity over field of view – fixed and stable, no alignment necessary
- Long lifetime
- Removable inline excitation filter holders – no moving parts
- Individual channel triggering via TTL in microseconds
- Sequence Runner – sequenced excitation from a single TTL-out
The
pE-300 range has three models-
Suitable for most
Leica,
Olympus,
Nikon and
Zeiss microscopes- please ask us about the adapters needed.
