CoolLED pE-340fura- Fast, Controllable LED Illumination for Fura-2
SKU:
1414
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The pE-340fura is a bespoke LED Illuminator for Fura-2 ratiometric calcium imaging. The calcium imaging light source utilises the successful pE-300 Series platform, and also supports everyday fluorescence microscopy in a compact and affordable package.
Until recently, the response time of illumination systems for Fura-2 imaging has been limited to milliseconds due to mechanical switching of the wavelengths in arc lamp and monochromator systems. However, the new pE-340fura can be controlled via convenient BNC TTL connections for precise illumination control in as little as 20 microseconds.
Using the new pE-340fura LED Illumination System, less Fura-2 dye can be loaded into the cells whilst still maintaining the same measured calcium concentration and good signal-to-noise ratio. The reduction in required dye not only improves cell-viability due to reduced dye toxicity, but also results in a cost reduction per experiment.
Work by Sandrine Prost et al., from the University of Edinburgh, has shown that with independent wavelength controllable LED sources, signal-to-noise is dramatically improved over bulb systems and even over some available white broad-spectrum LED sources.
To download a data sheet click here- download data sheet
Suitable for most Leica, Olympus, Nikon and Zeiss microscopes- please ask us about the adapters needed.
High-speed acquisition
Until recently, the response time of illumination systems for Fura-2 imaging has been limited to milliseconds due to mechanical switching of the wavelengths in arc lamp and monochromator systems. However, the new pE-340fura can be controlled via convenient BNC TTL connections for precise illumination control in as little as 20 microseconds.
Improved cell viability and cost
Using the new pE-340fura LED Illumination System, less Fura-2 dye can be loaded into the cells whilst still maintaining the same measured calcium concentration and good signal-to-noise ratio. The reduction in required dye not only improves cell-viability due to reduced dye toxicity, but also results in a cost reduction per experiment.
Improved signal to noise
Work by Sandrine Prost et al., from the University of Edinburgh, has shown that with independent wavelength controllable LED sources, signal-to-noise is dramatically improved over bulb systems and even over some available white broad-spectrum LED sources.
Features-
- Dedicated 340 nm and 380 nm outputs for Fura-2 ratiometric calcium imaging
- Broad white output (435 nm – 645 nm) for imaging standard fluorophores
- Microsecond switching (340/380) for capturing high-speed events
- Stable LED illumination minimises noise below that of experiment, reducing false positives and increasing precision.
- Higher signal-to-noise gives cleaner images and data whilst requiring less Fura-2 dye, reducing toxicity and costs
- UV optimised optics delivers maximum irradiance on standard microscope configurations
- Individual channel triggering via TTL in microseconds
- Removable inline excitation filter holders: no moving parts for fast acquisition
- Sequence Runner – sequenced excitation from a single TTL-out
- Individual channel selection controls the level of excitation of each dye independently on a multi-stained sample
- Compatible with most imaging software for integrated control with imaging set up
- Precise irradiance control in 1% steps (0-100%): no ND filters required
Convenience
- Specify for existing single and multi-band filter sets: no need to buy new filters
- Simple to fit, simple to use: no alignment, a once only adjustment
- Wide range of microscope adaptors: fits most microscopes
To download a data sheet click here- download data sheet
Suitable for most Leica, Olympus, Nikon and Zeiss microscopes- please ask us about the adapters needed.
Shipping is by courier and takes typically 2-5 days but this can alter.
Please phone us on +44 (0)1284 789697 or email us for current delivery times.
Request a quotation for bulk orders by emailing sales@gtvision.co.uk
If you have any questions about this model, complete the form below or email us at sales@gtvision.co.uk
- Because we supply microscopes from a wide variety of brands and manufacturers, we can offer expert advice on which microscope model is best suited for your application and budget without bias towards a particular brand.
- We provide tailor-built systems to match your specific requirements and budget.
- Expert support pre and post purchase.- For a full system solution, we also supply microscope cameras and software (again from a wide choice of brands).
- We can retro-fit cameras and illumination systems, including modern LED fluorescence illuminators, to most models and ages of existing microscopes. We also have in-house engineering capabilities for particularly unique requirements.