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CoolLED pE-340fura- Fast, Controllable LED Illumination for Fura-2

Price Excludes VAT & Delivery

Possible delivery methods: Express UK Mainland 1-2 Working Days, Customer pickup, Standard Mainland UK – For UK Addresses (3-5 working days), UK Islands & Scottish Highlands, Mainland Europe EU, Channel Islands, Outside Mainland Europe, Republic of Ireland – Special Low Delivery Charges

Description

The pE-340fura is a bespoke LED Illuminator for Fura-2 ratiometric calcium imaging. The calcium imaging light source utilises the successful pE-300 Series platform, and also supports everyday fluorescence microscopy in a compact and affordable package.

The 340 nm and 380 nm LED illumination system provides the optimum excitation wavelengths for Fura-2-based calcium imaging, allowing high-precision, stable, high-throughput imaging with video-rate time resolution.
High-speed acquisition

Until recently, the response time of illumination systems for Fura-2 imaging has been limited to milliseconds due to mechanical switching of the wavelengths in arc lamp and monochromator systems.  However, the new pE-340fura can be controlled via convenient BNC TTL connections for precise illumination control in as little as 20 microseconds.

Improved cell viability and cost

Using the new pE-340fura LED Illumination System, less Fura-2 dye can be loaded into the cells whilst still maintaining the same measured calcium concentration and good signal-to-noise ratio.  The reduction in required dye not only improves cell-viability due to reduced dye toxicity, but also results in a cost reduction per experiment.

Improved signal to noise

Work by Sandrine Prost et al., from the University of Edinburgh, has shown that with independent wavelength controllable LED sources, signal-to-noise is dramatically improved over bulb systems and even over some available white broad-spectrum LED sources.

Features-

  • Dedicated 340 nm and 380 nm outputs for Fura-2 ratiometric calcium imaging
  • Broad white output (435 nm – 645 nm) for imaging standard fluorophores
  • Microsecond switching (340/380) for capturing high-speed events
  • Stable LED illumination minimises noise below that of experiment, reducing false positives and increasing precision.
  • Higher signal-to-noise gives cleaner images and data whilst requiring less Fura-2 dye, reducing toxicity and costs
  • UV optimised optics delivers maximum irradiance on standard microscope configurations
  • Individual channel triggering via TTL in microseconds
  • Removable inline excitation filter holders: no moving parts for fast acquisition
  • Sequence Runner – sequenced excitation from a single TTL-out
  • Individual channel selection controls the level of excitation of each dye independently on a multi-stained sample
  • Compatible with most imaging software for integrated control with imaging set up
  • Precise irradiance control in 1% steps (0-100%): no ND filters required
Convenience
  • Specify for existing single and multi-band filter sets: no need to buy new filters
  • Simple to fit, simple to use: no alignment, a once only adjustment
  • Wide range of microscope adaptors: fits most microscopes

 

To download a data sheet click here- download data sheet

 

Suitable for most Leica, Olympus, Nikon and Zeiss microscopes- please ask us about the adapters needed.

Additional information

Weight 4 kg
Brand

CoolLED

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Use this delivery method if you live in the Channel Islands. Delivery times 3-7 days typically.

No VAT will be charged on your purchase if the delivery address is to the Channel Islands.

All orders for stock items are dispatched within 2 working days using a 3-7 working day delivery service.

Please note, we regret that, because of the cost of delivery of items to your region, by selecting this delivery method, you agree that you will pay the difference in price (compared to UK rates) of any delivery charges for any replacement or repair within the warranty period. (If you do not wish to accept these terms please do not proceed with your order.)

All orders for stock items are dispatched within 2 working days using a 2-5 working day delivery service.

If the delivery charge is the same as the std mainland UK rate the condition below does not apply.

Please note if your delivery charge is higher than the std mainland UK rate that is because it is more expensive to deliver items weighing over 0.6Kg by courier to your region and the condition below therefore applies:

Please note, For any consignment weighing over 0.6Kg we regret that, because of the cost of delivery of items to your region, by selecting this delivery method, you agree that you will pay the difference in price (compared to std mainland UK rates) of any delivery charges for any replacement or repair within the warranty period. (If you do not wish to accept these terms please do not proceed with your order.)

Scottish Highlands means these postcodes as defined by our carriers: AB1-9/14/21-23/30-39/41-45/51-/56. IV1 – 56. KW1 – 14. KA27 – 28. PA20 – 39. PH11 – 40. FK19 – 21