pE-4000 CoolLED Fluorescence Illuminator- Broad Spectrum 16 LED’s for Cutting-Edge Research

Description

The pE-4000 is a powerful, flexible, controllable system for advanced research applications. It has a wavelength range of 365-770 nm to cover all the common fluorophores. This system is ideal if you don’t know what future direction your research will take, and you need something that will provide a full range of fluorescence excitation wavelengths.

Advanced control for cutting-edge research

Multiple levels of control are possible, from simple to advanced. A dual mode control pod gives immediate manual control in either White or Advanced modes, where individual LED channels can be individually switched on/off or irradiance modulated.

Alternatively, the USB interface and integration into major microscope software platforms allows complex software control.

TTL control enables capturing high-speed events (<1 ms). Since there is no shutter or filter wheel, without these moving parts, this reduced latency means speed is limited only by the camera. An expansion box is also available for added functionality with multiple BNC connections for remote analogue and TTL control.

For optogenetics applications, alongside the ability to include excitation filters to tune the wavelength to the opsin’s maximum activation peak, an internal function generator controls pulse timing and waveforms.

Improving cell viability

With the pE-4000, it is now possible to prevent artefacts and allow live samples to thrive in experiments >100 hours. Fine control of illumination timing and irradiance with TTL and the function generator minimises exposure, avoiding photobleaching and phototoxicity.

Matching diverse fluorophore requirements

Full filter compatibility and 256 wavelength combinations from 16 installed LEDs (365 nm -770 nm) covers all available fluorophores, which is ideal for multi-user facilities or evolving experimental needs.

Multi-channel experiments with no pixel shift

Up to four channels using distinct wavelengths can be captured with no mechanical movement, preventing pixel shift and allowing the direct comparison of data.

Features

Performance

• Broadest LED spectrum available: 365-770 nm, with 16 LEDs
• Four channel wavelength-grouping design matches most commercially available dual, triple, and quad filters
• Removable inline excitation filter holders: no moving parts for fast acquisition
• Internal function generator for electrophysiology and optogenetics applications
• TTL and USB interfaces with major imaging software packages allows incorporation into all experimental parameters
• Analogue inputs for dynamic irradiance control
• Individual control of selected LED wavelengths reduces background
• Rapid switching between LED wavelengths enables capture of high-speed events
• Instant on/off: No shutters required, no warm up or cool down
• Stable and repeatable: reliable and consistent results
• Precise irradiance control in 1% steps (0-100%): no ND filters required

Convenience

• Pre-sets allow lab manager to match white spectrum to existing filter cubes
• Simple to fit, simple to use: no alignment, a once only adjustment
• Wide range of microscope adaptors: fits most microscopes

Suitable for most Leica, Olympus, Nikon and Zeiss microscopes- please ask us about the adapters needed.